Part:BBa_K917014:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917014

We have tested the expression of CymA protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and cymA gene). Cell pellets were scanned prior to sonication and cymA transformed cells show slightly more intense colour, indicating higher concentration of haem in cells.

Haem staining results suggest CymA expression in E coli transformed with plac-cymA and plac-ccm. The additional band indicated by an arrow in lane 3 represents CymA protein.

User Reviews

UNIQ0d43f7a863f3742d-partinfo-00000000-QINU UNIQ0d43f7a863f3742d-partinfo-00000001-QINU